Rapid and Sensitive Electrochemical Detection of Escherichia coli in water using Cr-Au IDE-Porous Silicon Sensor

An efficient electrochemical sensor based on Cr-Au Inter Digitated Electrodes (IDE)-porous silicon has been developed to rapidly assess Escherichia coli (E. coli) bacteria in water. Coliform bacteria, particularly E. coli, contribute significantly to waterborne contamination, driven by overuse and insufficient cleanliness around water bodies. This work incorporates the fabrication of porous silicon, characterization, synthesis of bacterial dilutions and testing of the sensor in the presence of varying E. coli dilutions. The dilutions are prepared from the stock solution of bacterial concentrations and H2O2. The interaction of porous silicon with bacteria incubated in H2O2 leads to a change in potential across the electrodes in real time. The limit of detection and sensitivity for the sensor are 0.187 CFU/mL and 113mV.mL/CFU respectively. The response and recovery time of the sensor is 80 milliseconds and 90 milliseconds, respectively. Additionally, analysis such as repeatability and testing in tap water, Pseudomonas and Citrobacter are conducted. For a user-friendly output, the sensor has been interfaced with a signal conditioning circuit and a display. This prototype offers a quick and precise way to identify the quality of drinking water, making it a potential solution to the growing problems caused by water pollution.